polyclonal anti porcine igm antibody  (Bio-Rad)

Journal: Veterinary Immunology and Immunopathology

Article Title: Quantitative immunohistochemical assessment of IgA, IgM, IgG and antigen-specific immunoglobulin secreting plasma cells in pig small intestinal lamina propria

doi: 10.1016/j.vetimm.2014.05.014

Figure Lengend Snippet: Antibody source, dilution, and antigen retrieval for immunohistochemistry to assess swine PCs producing IgG (a), IgM (b) and IgA (c) and the sandwich immunohistochemical protocol (d) for the detection of anti-PCV2 antibody-producing PCs.

Article Snippet: Primary antibody , Rabbit polyclonal anti-porcine IgG (Novus Biologicals Europe); 1:40,000 in PBS + 20% normal goat serum; overnight at 5 ± 3 °C , Rabbit polyclonal anti-porcine IgM (Novus Biologicals Europe); 1:40,000 in PBS + 20% normal goat serum; overnight at 5 ± 3 °C , Goat polyclonal anti-porcine IgA (Novus Biologicals Europe); 1:4,000 in PBS + 1% BSA; overnight at 5 ± 3 °C , Anti-PCV2 monoclonal antibody (F217, provided by G. Allan, Belfast, UK); 1:200 in PBS; 37 °C for 2 hours.

Techniques: Immunohistochemistry, Immunohistochemical staining, Blocking Assay, Suspension